In metallic materials, the activation of one slip system increases the flow strength of other slip systems, which is phenomenon known as latent hardening. This latent hardening behavior has been understood by the “forest hardening” mechanism arising from mutual dislocation interactions at the continuum length scale. As the size of a sample decreases to the submicron scale, the interactions between dislocations become increasingly sparse, so plastic deformation is instead governed mainly by dislocation sources. In this paper, we use three-dimensional dislocation dynamics (DD) simulations to examine plastic deformation in single crystalline Cu micropillars subjected to two types of combined loading conditions: tension after torsion and torsion after tension. These combined loadings are then compared with simple tension and pure torsion, respectively. We find that there exists a transition from latent hardening to latent softening in 600nm samples undergoing tension after torsion. The systematic computational and theoretical model described here suggests explosive multiplication causes dislocation density to greatly increase, giving rise to latent softening in those micropillars under tension after torsion.,